Application of qPCR-based SARS-Cov-2 variant detection to compliment Next Generation Sequencing (NGS)

Carpenter, R.E., Vine, T., Sharma, R.
Publication date
May 2022
2022 American Association of Bioanalyses and College of Reproductive Biology Symposium
Austin, TX, United States
The coronavirus (SARS-CoV-2) is one of the deadliest infectious disease outbreaks in recent history. Several variants of SARS-CoV-2 have emerged and some variants have caused second and third waves of the deadly infection. Some variants may be resistant to available vaccines and therapies. World Health Organization (WHO) has declared it as Variants of Concern (VOC); Alpha (B.1.1.7), Beta (B.1.351), Gamma (P.1), Delta (B.1.617.2), and Omicron (B.1.1.529).

There continues to be a need for swift and cost-effective SARS CoV-2 variant detection and monitoring. Next Generation Sequence (NGS) has been instrumental in discovering new mutations but the technique is expensive and time-consuming for mass deployment. Real-time PCR is widely used for the diagnosis of SARS-CoV-2, and the technology can be deployed for mass surveillance of known mutations and variants.