Streamlining fungal diagnostics: Evaluation of a Direct-to-PCR extraction-free workflow for candida detection
Authors
Sadia Almasa, Rob E. Carpenter, Vaibhav K. Tamrakarc, Aditya Sharmac, Kamalpreet Suric, Salima Karkia, Katelyn Kysera, Randy Sroncea, Rahul Sharma
Publication date
May 22, 2025
Journal
Streamlining fungal diagnostics: Evaluation of a Direct-to-PCR extraction-free workflow for candida detection
Volume
113
Pages
1-9
Publisher
Elsevier
Description
Fungal infections are an escalating health threat, and delays from conventional nucleic acid extraction hinder timely diagnosis and treatment. This study evaluated the diagnostic performance of a novel extraction-free technology, Direct-to-PCR (D2P; Scienetix, Tyler, TX, USA), for the detection of clinically significant Candida species (C. albicans, C. glabrata, C. auris, C. parapsilosis, and C. tropicalis). …